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 Home > COMMUNITY > Getting Started

Getting Started in Justin Lab.

June 27, 2015

1. Lab information

Lab Homepage: http://glia.kist.re.kr

Intranet: http://ekist.re.kr

Contact: PI: C. Justin Lee: 02-958-6940 / 010-2825-7128, cjl@kist.re.kr

CNS office Chunga Lee: 02-958-6939 / 010-4099-9647

CFC office Woojung Choi: 02-958-7214 / 010-9013-0610

 

2. Be prompt for lab meetings

We have a lab meeting on Mon and a journal club on Fri from 9:00 AM. During this meeting we will discuss lab business and present your own data collected in the past week. Attendance for each meeting is required for all members, please be prompt.

 

3. Preparing for lab meetings

1. Lab meeting

-Organizer: Jeongyeon Kim

-9:00 AM, every Monday, L7 B1 seminar room

-Lab business and update for mouse status: notes by Hyunjeong Kang

-Full work

: Presentation for one’s recent main project

: One presenter at a time ordered by age

: Prepare as a Ph.D thesis defense for students and as a job talk for postdocs.

-Personal progress

: Presentation for one’s results in past 2 weeks and plans for next 2 weeks

: Each person is assigned for alternating weeks

-All files must be saved in the backup folder in ftp://161.122.102.159/

 

2. Journal Club

-Organizer: Jaekwang Lee

-9:00 AM, every Friday, L7 B1 seminar room

-Glance at journals: Jungmoo Lee

: Update the interesting journal articles in current issue from assigned journals

-Email alert: Jungmoo Lee

: Proposal and selection for the abstract of the most interesting paper for next week

-Journal club

: All participants should read the paper thoroughly before the journal club

: Presenter should prepare a ppt containing introduction, material and method, result (purpose, method, result and implication), and conclusion.

: Email sender and homepage update for the information of next journal club: Jaeyoung Jung

-All files must be saved in the backup folder in ftp://161.122.102.159/

 

4. Keeping the lab clean

Cleaning of your experimental environment is very important for your health as well as quality of the experimental results. Therefore we may ask you to clean up daily. In every morning: Right after coming to lab, you may need to check the status of lab bench.

1. Empty waste bottle.

2. Arrangement of all the stuffs in the right place such as pipettes.

3. Check floor and clean if any dirty stuffs.

4. If anything needs to be clean, clean up.

Monthly Cleaning:

In every Monday of last week after lab meeting we will clean all equipment and refrigerators. Because of limited space, at this time, you need to clean all the experimental stuffs nearby equipment and make a space in the refrigerators

 

5. Learning essential skills for experiments

Obtaining basic skills is essential for you to perform experiments properly

1) Pipetting (training session by Eppendorf representative)

2) Properly calculating molarity and normality

3) Weighing and making stock solutions using volumetric flasks

4) Learn to use experimental machines and equipment

-Centrifuge (Wuhyun Koh)

-Vortexer and stirrer (Yeonha Ju)

-pH-meter and osmo-meter (Minho Nam, Young Eun Han)

-Sonicator (Wuhyun Koh)

-Water-bath and heat block (Wuhyun Koh)

-Clean bench and fume hood (Heejung Chun)

-Incubator (Young Eun Han)

-Freezer (Each person in charge)

-Microscopy for dissection and confocal (Heeyoung Ahn)

-PCR machine (Moonsun Sa)

-Electrophoresis (gel runner and gel doc) (Heejung Chun)

-Nanodrop (Soojin Oh)

-Autoclave and dryer (Yunha Joo)

-Vibratome and puller (Jaekwang Lee)

-Cryosection (Minho Nam)

-Virus injector (Junsung Woo)

-Electroporator (Young Eun Han)

 

5) Maintaining research consumables for experiments

-Ordering (Jea Kwon)

-Tip and tube (microtube and PCR tube) (Yunha Ju, Sunpil Kim)

-70% Ethanol (Jaeyoung Jung)

-Media for cell culture and E.coli (Heeyoung Ahn)

-Agarose gel and agar plate

-Stock for drug, antibody, enzyme, virus and DNA

 

6) Public duties

-Genotyping: (Junsung Woo)

: Tail prep (Jungmoo Lee, Yunha Ju)

: Result should be reported by the next weaning time

: Each person is assigned of specific mouse lines

-Mouse maintaining: (Seung Eun Lee)

: Each person should take training course on animal experimentation

: Each person should be fully informed about housing, weaning and ear-punching of mice

-Cell culture (primary astrocyte and neuron culture, mammalian cell lines)

-shRNA cloning (using essential experimental techniques including oligomer design, PCR/RT-PCR, transformation, mini-prep, midi-prep, electrophoresis, DNA sequencing, cell culture, microroporation)

 

7) Experimental techniques

-Primary astrocyte culture (Sooyoung Jeong, Jea Kwon)

-Primary neuron culture (Heeyoung Ahn)

-Cell-line culture (Joungha Won)

-3D brain culture (Soojin Oh)

-Calcium imaging (Jungmoo Lee)

-FRET imaging (Junsung Woo)

-Two-photon imaging (Jaekwang Lee)

-Cultured cell patch clamp recording (Soojin Oh, Young Eun Han)

-Sniffer patch clamp recording (Soojin Oh)

-Pressure clamp recording (Young Eun Han)

-Slice patch clamp recording (Jaekwang Lee, Junsung Woo)

-Slice field recording (Wuhyun Koh)

-MEA recording (Soojin Oh)

-Autopatch (Jea Kwon)

-Oocyte recording (Moonsun Sa)

-Immunohistochemistry (Heeyoung Ahn)

-Behavior tests (Jeongyeon Kim)

-Microdialysis (Jeongyeon Kim)

-HPLC analysis (Jeongyeon Kim)

-Optogenetic stimulation (Jeongyeon Kim)

-Brain infusion with micropump (Jeongyeon Kim)

-Virus injection (Junsung Woo)

-SNP analysis (Junsung Woo)

-DNA cloning (Heejung Chun)

-RNA work (Heejung Chun)

-qRT-PCR (Young Eun Han)

-Western blot (Soojin Oh)

 

You can find more protocols from the backup folder of our lab (ftp://161.122.102.159/backup/protocol/)

8) Backing up data and presentation files (Junsung Woo)

-ftp://161.122.107.43/

-ftp://161.122.107.48/

-Each person needs username and password for access.

-All rawdata, analysis results, and presentations files must be backed up in these public drives

-Backing up data and presentation files (Junsung Woo)

9) Lab home page maintenance (Wuhyun Koh)

http://glia.kist.re.kr

Update your personal info

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Room 7223, Building L7, Division of Life Sciences, KIST, 39-1 Hawolgokdong, Seongbukgu, Seoul, 136-791 Republic of Korea
Tel: +82-2-958-6421 Fax: +82-2-958-6919 E-mail: cjl@kist.re.kr
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