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Getting Started in Justin Lab.

February 5, 2018

1. Lab information

Lab Homepage: http://glia.kist.re.kr

Intranet: http://ekist.re.kr

Contact: PI: C. Justin Lee: 02-958-6940 / 010-2825-7128, cjl@kist.re.kr
Junior PI: Soo-Jin Oh: 010-7272-7535, osj@kist.re.kr
Glia office: Yeonju Kim: 02-958-7215 / 010-9787-0089
CNS office: Eunmi Hong: 02-958-6939 / 010-2931-6450

2. Be prompt for lab meetings

We have a lab meeting on Mon and a journal club on Fri from 9:00 AM. During this meeting we will discuss lab business and present your own data collected in the past week. Attendance for each meeting is required for all members, please be prompt.

3. Preparing for lab meetings

1) Lab meeting

-Organizer: Min-ho Nam

-9:00 AM, every Monday, L7 B1 seminar room

-Lab business and update for mouse status: notes by Sunpil Kim

-Full work

-Personal progress
: Presentation for one's recent main project
: One presenter at a time ordered by age
: Prepare as a Ph.D thesis defense for students and as a job talk for postdocs

-Personal progress
: Presentation for one's results in past 2 weeks and plans for next 2 weeks
: Each person is assigned for alternating weeks

-All files must be saved in the backup folder in

2) Journal Club

-Organizer: Heeyoung An

-9:00 AM, every Friday, L7 B1 seminar room

-Glance at journals: Mingu Park
: Update the interesting journal articles in current issue from assigned journals

-Email alert: Mingu Park
: Proposal and selection for the abstract of the most interesting paper for next week

-Journal club
: All participants should read the paper thoroughly before the journal club
: Presenter should prepare a ppt containing introduction, material and method, result (purpose, method, result and implication), and conclusion.
: Email sender and homepage update for the information of next journal club:
Min-woo Jang

-All files must be saved in the backup folder in

4. Keeping the lab clean

Cleaning of your experimental environment is very important for your health as well as quality of the experimental results. Therefore we may ask you to clean up daily. In every morning: Right after coming to lab, you may need to check the status of lab bench.

1) Empty waste bottle.

2) Arrangement of all the stuffs in the right place such as pipettes.

3) Check floor and clean if any dirty stuffs.

4) If anything needs to be clean, clean up.

5) Monthly Cleaning:

In every Monday of last week after lab meeting we will clean all equipment and refrigerators. Because of limited space, at this time, you need to clean all the experimental stuffs nearby equipment and make a space in the refrigerators

5.Learning essential skills for experiments
Obtaining basic skills is essential for you to perform experiments properly

1) Pipetting (training session by Eppendorf representative)

2) Properly calculating molarity and normality

3) Weighing and making stock solutions using volumetric flasks

4) Learn to use experimental machines and equipment

-Centrifuge (Moonsun Sa)

-Vortexer and stirrer (Yeonha Ju)

-pH-meter and osmo-meter (Mingu Park)

-Sonicator (Wuhyun Koh)

-Water-bath and heat block (Wuhyun Koh)

-Clean bench and fume hood (Min-woo Jang)

-Perfusion clean bench and fume hood (Jeong-im Shin)

-Incubator (Youngeun Han, Joungha Won)

-Deep freezer (Moonsun Sa)

-Enzyme freezer (Yeonha Ju)

-Freezer (Each person in charge)

-Upright Microscopy (Min-ho Nam)

-Inverted Microscopy (Jea Kwon)

-Microscopy for confocal (Heeyoung An)

-PCR machine (Moonsun Sa)

-Electrophoresis gel runner (Jeong-im Shin, Yeonha Ju)

-Electrophoresis gel doc (Moonsun Sa)

-Nanodrop (Joungha Won)

-Vibratome and slice patch puller (Jea Kwon, Woojin Won)

-Cell patch puller (Min-woo Jang)

-Cryosection (Heeyoung An)

-Virus injector (Sunpil Kim)

-Electroporator (Youngeun Han)

-Distilled water purifier (Jungmoo Lee)

-Heat block (Yongmin Park)

-37℃ Incubator for molecular assay (Min-woo Jang)

5) Maintaining research consumables for experiments

-Ordering (Joungha Won)

-Tip and tube (microtube and PCR tube) (Yongmin Park)

-70% Ethanol (Yongmin Park)

-Media for cell culture and E.coli (Yeonha Ju)

-Agarose gel and agar plate

-Stock for drug, antibody, enzyme, virus and DNA

6) Public duties

-Genotyping: (Heejung Chun)
: Tail prep
: Result should be reported by the next weaning time
: Each person is assigned of specific mouse lines

-Mouse maintaining: (Kyoungye Ryu)
: Each person should take training course on animal experimentation
: Each person should be fully informed about housing, weaning and ear-punching of mice

-Cell culture (primary astrocyte and neuron culture, mammalian cell lines)

-shRNA cloning (using essential experimental techniques including oligomer design, PCR/RT-PCR, transformation, mini-prep, midi-prep, electrophoresis, DNA sequencing, cell culture, microroporation)

7) Experimental techniques

-Primary astrocyte culture (Heeyoung An, Mingu Park, Jeong im Shin)

-Primary neuron culture (Yeonha Ju)

-Cell-line culture (Joungha Won)

-3D brain culture (Soojin Oh)

-Calcium imaging (Jungmoo Lee)

-FRET imaging (Youngeun Han)

-Two-photon imaging (Jungmoo Lee, Sunpil Kim)

-Cultured cell patch clamp recording (Jungmoo Lee, Min-woo Jang, Youngeun Han)

-Sniffer patch clamp recording (Jungmoo Lee, Wuhyun Koh)

-Pressure clamp recording (Youngeun Han)

-Slice patch clamp recording (Jea Kwon, Woojin Won, Joungha Won, Moonsun Sa)

-Slice field recording (Wuhyun Koh)

-MEA recording (Soojin Oh)

-Autopatch (Jea Kwon)

-Immunohistochemistry (Heeyoung An)

-Behavior tests (Sunpil Kim)

-Microdialysis (Sunpil Kim)

-HPLC analysis (Sunpil Kim)

-Optogenetic stimulation (Joungha Won)

-Brain infusion with micropump (Sunpil Kim)

-Virus injection (Min-ho Nam)

-SNP analysis (Joungha Won)

-DNA cloning (Joungha Won)

-RNA work (Min-woo Jang)

-qRT-PCR (Joungha Won)

-Western blot (Sunpil Kim, Mingu Park, Min-woo Jang, Soojin Oh)

-MAOB, DAO action assay (Wuhyun Koh)

-8 arm radial maze (Moonsun Sa)

-Single unit recording (Mingu Park)

-Confocal microscopy (Heeyoung An)

You can find more protocols from the backup folder of our lab

8) Backing up data and presentation files (Wuhyun Koh)



-Each person needs username and password for access.

-All rawdata, analysis results, and presentations files must be backed up in these public drives

9) Lab home page maintenance (Wuhyun Koh)

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Room 7223, Building L7, Division of Life Sciences, KIST, 39-1 Hawolgokdong, Seongbukgu, Seoul, 136-791 Republic of Korea
Tel: +82-2-958-6421 Fax: +82-2-958-6919 E-mail: cjl@kist.re.kr
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